388 The impact of the susceptibility gene NOS2 and its bioactive molecule Nitric oxide in the pathogenesis of Psoriasis

Psoriasis is an immune-mediated systemic skin disorder with a strong genetic predisposition. The gene expression of the psoriasis susceptibility Nitric oxide synthase 2 (NOS2) specifically upregulated in psoriatic involved skin. NOS2 catalyses synthesis nitric (NO), short-lived free radical and pivotal signaling molecule both pro- anti-inflammatory properties. In this study, we have investigated functional relevance psoriasis. Using qPCR, found that mRNA was strongly expressed epidermis compared to healthy Surprisingly, corresponding increase protein not detected using immunohistochemistry. We psoriasis-associated miRNAs miR-31 miR-146a prevent full translation keratinocytes demonstrated effect IL-17 dependent. Hence, our data suggest NOS2-derived NO likely be present at low levels imiquimod (IMQ) mouse model, histological characteristics inflammation were distinctly reduced knockout mice. Furthermore, neutrophilic infiltrate cell proliferation markers Ki67 PCNA detected. Our imply presence response contribute wild type conclusion, induces fine tunes its towards window proinflammatory hyperproliferative effects. Based on these findings, expect would alleviate phenotype.